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A Java framework for statistical analysis and classification of biological sequences

Sequence analysis is one of the major subjects of bioinformatics. Several existing libraries combine the representation of biological sequences with exact and approximate pattern matching as well as alignment algorithms. We present Jstacs, an open source Java library, which focuses on the statistical analysis of biological sequences instead. Jstacs comprises an efficient representation of sequence data and provides implementations of many statistical models with generative and discriminative approaches for parameter learning. Using Jstacs, classifiers can be assessed and compared on test datasets or by cross-validation experiments evaluating several performance measures. Due to its strictly object-oriented design Jstacs is easy to use and readily extensible.

Jstacs is a joint project of the groups Bioinformatics and Pattern Recognition and Bioinformatics at the Institute of Computer Science of Martin Luther University Halle-Wittenberg and the Bioinformatics group of the Julius Kuehn Institute. Initially the projects has also been developed at the Leibniz Institute of Plant Genetics and Crop Plant Research.

Jstacs is listed in the machine learning open-source software (mloss) repository.

Licensing Information

Jstacs is free software: you can redistribute it and/or modify under the terms of the GNU General Public License version 3 or (at your option) any later version as published by the Free Software Foundation.

Current release

You can download Jstacs version 2.2 here.
You find an overview of the new features in the Version history.
We also provide an API documentation, a Cookbook, and a Reference card for this release.

Getting started & Cookbook

For set-up instructions, a list of basic requirements, and suggestions for your first steps with Jstacs, please see Getting started.

Since version 2.0, we offer a Cookbook for Jstacs in addition to the API documentation. This cookbook comprises a general description of the structure of Jstacs including data handling, statistical models, classifiers, and assessments. The cookbook is accompanied by a number of Recipes or Code examples that can serve as a starting point of your own applications.

For a quick reference, we also provide a Reference card.


The paper about Jstacs has been published in the Journal of Machine Learning Research. If you use Jstacs in your research, please cite

J. Grau, J. Keilwagen, A. Gohr, B. Haldemann, S. Posch, and I. Grosse. Jstacs: A java framework for statistical analysis and classification of biological sequences. Journal of Machine Learning Research, 13(Jun):1967–1971, 2012.

BibTeX entry


Applications currently using Jstacs:

Bug reports & Feature requests

You can submit bug reports and feature requests by mail to

Latest Papers

The paper AnnoTALE: bioinformatics tools for identification, annotation, and nomenclature of TALEs from Xanthomonas genomic sequences has been published in Scientific Reports.

The paper Using intron position conservation for homology-based gene predictions has been published in Nucleic Acids Research.

The paper Inferring intra-motif dependencies of DNA binding sites from ChIP-seq data has been published in BMC Bioinformatics.

The paper Varying levels of complexity in transcription factor binding motifs has been published in Nucleic Acids Research.

The paper Area under Precision-Recall Curves for Weighted and Unweighted Data has been published in PLOS ONE.

The paper A general approach for discriminative de-novo motif discovery from high-throughput data has been published in Nucleic Acids Research.

Further papers and projects can be found under Projects.

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